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Polymers For Advanced Technologies Dec 2020A novel antimicrobial dental self-cured glass-ionomer cement has been developed and evaluated. Alumina filler particles were covalently coated with an antibacterial...
A novel antimicrobial dental self-cured glass-ionomer cement has been developed and evaluated. Alumina filler particles were covalently coated with an antibacterial polymer and blended into a self-cured glass-ionomer cement formulation. Surface hardness and bacterial viability were used to evaluate the modified cements. Results showed that the modified cements exhibited a significantly enhanced antibacterial activity along with improved surface hardness. Effects of antibacterial moiety content, alumina particle size and loading, and total filler content were investigated. It was found that increasing antibacterial moiety content, particle size and loading, and total filler content generally increased surface hardness. Increasing antibacterial moiety, filler loading and total filler content increased antibacterial activity. On the other hand, increasing particle size showed a negative impact on antibacterial activity. The leaching tests indicate no cytotoxicity produced from the modified cements to both bacteria and 3T3 mouse fibroblast cells.
PubMed: 35634167
DOI: 10.1002/pat.5029 -
Oxidative Medicine and Cellular... 2017The generation of reactive oxygen and nitrogen species (RONS) has been found to occur during inflammatory procedures, during cell ischemia, and in various crucial... (Review)
Review
The generation of reactive oxygen and nitrogen species (RONS) has been found to occur during inflammatory procedures, during cell ischemia, and in various crucial developmental processes such as cell differentiation and along cell signaling pathways. The most common sources of intracellular RONS are the mitochondrial electron transport system, NADH oxidase, and cytochrome P450. In this review, we analyzed the extracellular and intracellular sources of reactive species, their cell signaling pathways, the mechanisms of action, and their positive and negative effects in the dental field. In dentistry, ROS can be found-in lasers, photosensitizers, bleaching agents, cold plasma, and even resin cements, all of which contribute to the generation and prevalence of ROS. Nonthermal plasma has been used as a source of ROS for biomedical applications and has the potential for use with dental stem cells as well. There are different types of dental stem cells, but their therapeutic use remains largely untapped, with the focus currently on only periodontal ligament stem cells. More research is necessary in this area, including studies about ROS mechanisms with dental cells, along with the utilization of reactive species in redox medicine. Such studies will help to provide successful treatment modalities for various diseases.
Topics: Cytochrome P-450 Enzyme System; Humans; Lasers; Low-Level Light Therapy; NADPH Oxidases; Oxidative Stress; Photosensitizing Agents; Plasma Gases; Reactive Nitrogen Species; Reactive Oxygen Species; Stem Cells; Tooth Diseases
PubMed: 29204250
DOI: 10.1155/2017/7542540 -
Biomaterials Sep 2021Injectable polymers have attracted intensive attention in tissue engineering and drug delivery applications. Current injectable polymer systems often require...
Injectable polymers have attracted intensive attention in tissue engineering and drug delivery applications. Current injectable polymer systems often require free-radical or heavy-metal initiators and catalysts for the crosslinking process, which may be extremely toxic to the human body. Here, we report a novel polyhedral oligomeric silsesquioxane (POSS) based strain-promoted alkyne-azide cycloaddition (SPAAC) "click" organic-inorganic nanohybrids (click-ON) system that can be click-crosslinked without any toxic initiators or catalysts. The click-ON scaffolds supported excellent adhesion, proliferation, and osteogenesis of stem cells. In vivo evaluation using a rat cranial defect model showed outstanding bone formation with minimum cytotoxicity. Essential osteogenic alkaline phosphatase (ALP) and vascular CD31 marker expression were detected on the defect site, indicating excellent support of in vivo osteogenesis and vascularization. Using salt leaching techniques, an injectable porous click-ON cement was developed to create porous structures and support better in vivo bone regeneration. Beyond defect filling, the click-ON cement also showed promising application for spinal fusion using rabbits as a model. Compared to the current clinically used poly (methyl methacrylate) (PMMA) cement, this click-ON cement showed great advantages of low heat generation, better biocompatibility and biodegradability, and thus has great potential for bone and related tissue engineering applications.
Topics: Animals; Bone Cements; Bone Regeneration; Hydrogels; Osteogenesis; Rabbits; Rats; Tissue Engineering; Tissue Scaffolds
PubMed: 34280821
DOI: 10.1016/j.biomaterials.2021.121014 -
Heliyon Aug 2020The article presents classification of the thermosetting materials for bone augmentation. The physical, mechanical, biological, and clinical properties of such materials... (Review)
Review
The article presents classification of the thermosetting materials for bone augmentation. The physical, mechanical, biological, and clinical properties of such materials are reviewed. There are two main types of curable osteoplastic materials: bone cements and hydrogels. Compared to hydrogels, bone cements have high strength features, but their biological properties are not ideal and must be improved. Hydrogels are biocompatible and closely mimic the extracellular matrix. They can be used as cytocompatible scaffolds for tissue engineering, as can protein- and nucleic acid-activated structures. Hydrogels may be impregnated with osteoinductors such as proteins and genetic vectors without conformational changes. However, the mechanical properties of hydrogels limit their use for load-bearing bone defects. Thus, improving the strength properties of hydrogels is one of the possible strategies to achieve the basis for an ideal osteoplastic material.
PubMed: 32817899
DOI: 10.1016/j.heliyon.2020.e04686 -
Journal of Clinical Medicine Jan 2024Symptomatic osteoporotic compression fractures are commonly addressed through vertebroplasty and kyphoplasty. However, cement leakage poses a significant risk of...
Symptomatic osteoporotic compression fractures are commonly addressed through vertebroplasty and kyphoplasty. However, cement leakage poses a significant risk of neurological damage. We introduced "aspiration percutaneous kyphoplasty", also known as "decompressed kyphoplasty", as a method to mitigate cement leakage and conducted a comparative analysis with high viscosity cement vertebroplasty. We conducted a retrospective study that included 136 patients with single-level osteoporotic compression fractures. Among them, 70 patients underwent high viscosity cement vertebroplasty, while 66 patients received decompressed percutaneous kyphoplasty with low-viscosity cement. Comparison parameters included cement leakage rates, kyphotic angle alterations, and the occurrence of adjacent segment fractures. The overall cement leakage rate favored the decompressed kyphoplasty group (9.1% vs. 18.6%), although statistical significance was not achieved ( = 0.111). Nonetheless, the risk of intradiscal leakage significantly reduced in the decompressed kyphoplasty cohort ( = 0.011), which was particularly evident in cases lacking the preoperative cleft sign on X-rays. Kyphotic angle changes and the risk of adjacent segment collapse exhibited similar outcomes ( = 0.739 and 0.522, respectively). We concluded that decompressed kyphoplasty demonstrates efficacy in reducing intradiscal cement leakage, particularly benefiting patients without the preoperative cleft sign on X-rays by preventing intradiscal leakage.
PubMed: 38256479
DOI: 10.3390/jcm13020345 -
Heliyon Jan 2021It would certainly be an advantage of resin-modified glass ionomer cement (RMGIC) if it can possess bioactivity. However, research related to that is still very limited....
It would certainly be an advantage of resin-modified glass ionomer cement (RMGIC) if it can possess bioactivity. However, research related to that is still very limited. Hybridization of RMGIC was predicted to increase mechanical properties and resistance to disintegration, and low content of polyacrylic acid induces cement bioactivity. This study investigated the effect of BAG obtained from the CaSiO-Ca(PO) system on RMGIC bioactivity. BAG samples containing 10%, 15%, and 20% PO (denoted as "CSP10," "CSP15," and "CSP20," respectively) were used to modify RMGIC powder, and apatite wollastonite was used for comparison. Surface bioactivity was assessed using XRD pattern, infrared spectroscopy, and SEM microstructure after specimen immersion in simulated body fluid (SBF). Contents of Ca, P, F, Sr, and Al were measured in the remaining SBF. Cell attachment, proliferation, and differentiation on the RMGIC containing BAG were evaluated and compared with those on the RMGIC without BAG. Sr and Al analyses revealed that the addition of BAG may not influence the matrix stability of the cement. Moreover, the addition of BAG was a positive factor indicating excellent ion exchange in SBF and spontaneous growth of apatite by consuming the Ca and P ions in the surrounding fluid. Osteoblast differentiation was higher on the four types of bioactive cements than on the RMGIC without BAG. The results of these studies provide novel insights into the development of a new generation of osteoconductive biomedical materials.
PubMed: 33506127
DOI: 10.1016/j.heliyon.2021.e05944 -
Bioengineering (Basel, Switzerland) Jul 2020Polymeric materials are the first choice for restoring tooth cavities, bonding tooth-colored fillings, sealing root canal systems, and many other dental restorative... (Review)
Review
Polymeric materials are the first choice for restoring tooth cavities, bonding tooth-colored fillings, sealing root canal systems, and many other dental restorative applications. However, polymeric materials are highly susceptible to bacterial attachment and colonization, leading to dental diseases. Many approaches have been investigated to minimize the formation of biofilms over polymeric restorative materials and at the tooth/material interfaces. Among them, contact-killing compounds have shown promising results to inhibit dental biofilms. Contact-killing compounds can be immobilized within the polymer structure, delivering a long-lasting effect with no leaching or release, thus providing advantages compared to release-based materials. This review discusses cutting-edge research on the development of contact-killing compounds in dental restorative materials to target oral pathogens. Contact-killing compounds in resin composite restorations, dental adhesives, root canal sealers, denture-based materials, and crown cements have all demonstrated promising antibacterial properties. Contact-killing restorative materials have been found to effectively inhibit the growth and activities of several oral pathogens related to dental caries, periodontal diseases, endodontic, and fungal infections. Further laboratory optimization and clinical trials using translational models are needed to confirm the clinical applicability of this new generation of contact-killing dental restorative materials.
PubMed: 32751652
DOI: 10.3390/bioengineering7030083 -
Materials (Basel, Switzerland) Mar 2017Calcium phosphate (CaP) bioceramics are widely used in the field of bone regeneration, both in orthopedics and in dentistry, due to their good biocompatibility,... (Review)
Review
Calcium phosphate (CaP) bioceramics are widely used in the field of bone regeneration, both in orthopedics and in dentistry, due to their good biocompatibility, osseointegration and osteoconduction. The aim of this article is to review the history, structure, properties and clinical applications of these materials, whether they are in the form of bone cements, paste, scaffolds, or coatings. Major analytical techniques for characterization of CaPs, in vitro and in vivo tests, and the requirements of the US Food and Drug Administration (FDA) and international standards from CaP coatings on orthopedic and dental endosseous implants, are also summarized, along with the possible effect of sterilization on these materials. CaP coating technologies are summarized, with a focus on electrochemical processes. Theories on the formation of transient precursor phases in biomineralization, the dissolution and reprecipitation as bone of CaPs are discussed. A wide variety of CaPs are presented, from the individual phases to nano-CaP, biphasic and triphasic CaP formulations, composite CaP coatings and cements, functionally graded materials (FGMs), and antibacterial CaPs. We conclude by foreseeing the future of CaPs.
PubMed: 28772697
DOI: 10.3390/ma10040334 -
Journal of Bone Oncology Feb 2021Metastatic bone lesions are common among patients with advanced cancers. While chemotherapy and radiotherapy may be prescribed immediately after diagnosis, the majority... (Review)
Review
Metastatic bone lesions are common among patients with advanced cancers. While chemotherapy and radiotherapy may be prescribed immediately after diagnosis, the majority of severe metastatic bone lesions are treated by reconstructive surgery, which, in some cases, is followed by postoperative radiotherapy or chemotherapy. However, despite recent advancements in orthopedic surgery, patients undergoing reconstruction still have the risk of developing severe complications such as tumor recurrence and reconstruction failure. This has led to the introduction and evaluation of poly (methyl methacrylate) and inorganic bone cements as local carriers for chemotherapeutic drugs (usually, antineoplastic drugs (ANPDs)). The present work is a critical review of the literature on the potential use of these cements in orthopedic oncology. While several studies have demonstrated the benefits of providing high local drug concentrations while minimizing systemic side effects, only six studies have been conducted to assess the local toxic effect of these drug-loaded cements and they all reported negative effects on healthy bone structure. These findings do not close the door on chemotherapeutic bone cements; rather, they should assist in materials selection when designing future materials for the treatment of metastatic bone disease.
PubMed: 33552885
DOI: 10.1016/j.jbo.2020.100345 -
International Journal of Molecular... Jul 2016Glass ionomer cements (GICs) are being used for a wide range of applications in dentistry. In order to overcome the poor mechanical properties of glass ionomers, several... (Review)
Review
Glass ionomer cements (GICs) are being used for a wide range of applications in dentistry. In order to overcome the poor mechanical properties of glass ionomers, several modifications have been introduced to the conventional GICs. Nanotechnology involves the use of systems, modifications or materials the size of which is in the range of 1-100 nm. Nano-modification of conventional GICs and resin modified GICs (RMGICs) can be achieved by incorporation of nano-sized fillers to RMGICs, reducing the size of the glass particles, and introducing nano-sized bioceramics to the glass powder. Studies suggest that the commercially available nano-filled RMGIC does not hold any significant advantage over conventional RMGICs as far as the mechanical and bonding properties are concerned. Conversely, incorporation of nano-sized apatite crystals not only increases the mechanical properties of conventional GICs, but also can enhance fluoride release and bioactivity. By increasing the crystallinity of the set matrix, apatites can make the set cement chemically more stable, insoluble, and improve the bond strength with tooth structure. Increased fluoride release can also reduce and arrest secondary caries. However, due to a lack of long-term clinical studies, the use of nano-modified glass ionomers is still limited in daily clinical dentistry. In addition to the in vitro and in vivo studies, more randomized clinical trials are required to justify the use of these promising materials. The aim of this paper is to review the modification performed in GIC-based materials to improve their physicochemical properties.
Topics: Ceramics; Dental Cements; Glass Ionomer Cements; Humans; Nanoparticles
PubMed: 27428956
DOI: 10.3390/ijms17071134